5th Training School, Valencia (Spain), March 2016

Cryopreservation of fish germ cells
Valencia (Spain), 7th-11th March, 2016

Scientific report

The 5th AQUAGAMETE Training School took place from 7th to 11th of March, 2016, in the facilities of the Universitat Politècnica de València and PROiSER S.L. (Spain). The course was organized by Juan F. Asturiano, Chair of this Action, and his group. A total of 21 trainees from 10 different countries attended the course, plus some local students who attended as auditors.
Theoretical lectures dealt about different topics related to cryopreservation and were given by 9 experts. Practical classes aimed to train students on techniques for sperm cryopreservation, microinjection of embryos and larvae, isolation of spermatogonia, and sperm quality evaluation by CASA.

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Two technical visits were performed, one to the Albufera lagoon and surroundings, where participants enjoyed a boat ride with stops to visit the Tancat de la Pipa (environment preservation center), the Center for Preservation of Freshwater Species (Generalitat Valenciana) and the fishermen facilities in El Palmar, where they were invited to a talk about European eel fishing in Albufera lagoon. After a typical lunch in the village, they visited the Estany, Gola Pujol and Saler beach (natural exit way of migratory eels). The second technical visit was to PROiSER S.L., a company focused on research and development of automatic diagnostic systems, especially for sperm quality evaluation. There, participants were taught about CASA system and its use.

Juan F. Asturiano

Program:

You can see the videos and presentations by clicking on the link below the title of each talk.

Monday, March 7th
09:00-09:30 Registration and welcome
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=c810d497-5840-4ad8-a83d-95aa326dac0c
09:30-10:00 Progress, challenges and perspectives on fish gamete cryopreservation. J.F. Asturiano (UPV, Spain).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=1ff34016-0957-4cd9-b392-88c52806fd93
Presentation: Slides Juan F Asturiano
10:00-10:30 Basic info on cryopreservation process. Á. Horváth (SIU, Hungary).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=35e66dbf-0079-43e2-8547-d5f9bd1887eb
Presentation: Slides Ákos Horváth
10:30-11:00 Coffee break.
11:00-11:30 Surrogate production technology in fish. M. Psenicka (USB, Czech Republic).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=efac8aeb-58a9-42fa-a046-df4e7e10677a
Presentation: Slides Martin Psenicka
11:30-12:25 IMPRESS ESRs presentations (7 x 8 min).
12:25-12:40 Break.
12:40-13:45 IMPRESS ESRs presentations (8 x 8 min).
Presentation: Slides Elia Ciani
13:45-15:00 Lunch.
15:00-18:00 Practical 1: Sperm cryopreservation in marine fish. David S. Peñaranda + UPV team.

Tuesday, March 8th
09:00-18:00 Technical visits:
Puerto de Catarroja,
Tancat de la Pipa,
Albufera lagoon,
Center for preservation of freshwater species CV (CCEDCV)-El Palmar,
Fishermen facilities El Palmar and talk by M. Jover (UPV, Spain): Eel fishing in Albufera lake.
Estany, Gola Pujol and Saler beach.

Wednesday, March 9th
08:15-09:00 Somatic cells as an additional resource for genome preservation: the promises and the difficulties. C. Labbé (INRA, France).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=047fa3c7-1d0a-42dc-bd39-70f11643a229
Presentation: Slides Catherine Labbé
09:00-11:00 Practical 2, part I: Microinjection of ZF embryos. M. Psenicka (USB, Czech Republic) + UPV team.
11:00-11:30 Coffee break.
11:30-13:00 Practical 2, part II: Transplantation of blastomeres from injected zebrafish embryos M. Psenicka (USB, Czech Republic) + UPV team.
13:00-14:30 Lunch.
14:30-15:15 Epigenetics and non-Mendelian inheritance. F. Piferrer (CSIC, Spain).
15:15-16:00 Fish PGC cryopreservation: is it a realistic tool for fish conservation biology? V. Robles (IEO, Spain).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=d58e7ad4-cdec-44f6-9662-5d764508c553
Presentation: Slides Vanesa Robles

Thursday, March 10th
09:00-12:00 Practical 3: Isolation and transplantation of sturgeon spermatogonia into sturgeon larvae. M. Psenicka (USB, Czech Republic) + UPV team.
12:00-12:30 Coffee break.
12:30-13:15 Standardization and reporting in cryopreservation. Á. Horváth (SIU, Hungary).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=61472ec2-d94d-4ae9-b7d4-efec7f2eece1
Presentation: Slides Akos Horvath Standardization
13:15-13:45 Case study 1: Gonia cryopreservation (sturgeon). M. Psenicka (USB, Czech Republic).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=352102d3-7df5-4dcb-bf7e-e81d00e07b66
13:45-15:15 Lunch.
15:15-15:45 Case study 2: European eel sperm cryopreservation. L. Pérez (UPV, Spain).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=0cc5187f-e0e2-4c44-a7c0-500896f4866e
Presentation: Slides Luz Pérez
15:45-16:15 Case study 3: Salmon sperm cryopreservation (Efficient method for cryopreservation of salmonid fish semen). A. Ciereszko (PAN, Poland).
Video: https://engage.videoapuntes.upv.es/paella4.0/ui/watch.html?id=72b8acc4-a45a-428b-bab9-cccf0455463a
Presentation: Slides Andrzej Ciereszko
16:15-17:05 AQUAGAMETE ESRs presentations
Presentations: Slides Sude Atmaca, Slides Pietro Boccaletto, Slides Jorge Hernández Urcera,  Slides Eszter KasaSlides Agnes Osz, Slides Taina Rocha AlmeidaSlides Momin Siddique

Friday, March 11th
09:00-11:00 Technical visit PROISER facilities. C. Soler and PROISER team (PROISER, Spain).
11:00-11:30 Coffee break.
11:30-13:30 Practical 4: Sperm quality evaluation (CASA). C. Soler and PROISER team       (PROISER, Spain) / AQUAGAMETE Core Group + WG2 meetings.
13:30-15:00 Lunch (UV Campus).
15:00-18:00 Airport/Campus.

Second announcement

Valencia, January 18th, 2016

Dear colleagues,

More detailed information is now available for the 5th AQUAGAMETE Training School which will be held at the Universitat Politècnica de València and PROiSER R+D facilities, Spain.

Please visit http://trainingschoolvalencia.weebly.com/, where you will find specific and practical information about the event, including a link to the registration form.

We take the chance to remind you that the application deadline is February 5th. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by February 10th. Please do not register until you have been notified that were selected as trainee. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com). Applications should include:

  • Application form.
  • Short Curriculum Vitae in the area.
  • Motivation letter for participating in the training school, addressed to Dr. Juan F. Asturiano and indicating the academic/professional status of the applicant.
  • Letter of recommendation from the applicant’s supervisor or employer.
  • Financial Support justification (brief explanation of expected travel costs derived of the attendance to the Training School, especially in travel, accommodation and meals).

Please make sure that your name is present in every document.

Financial support from the AQUAGAMETE COST Action (for travelling, accommodation and meals) will be available for selected trainees. The amount of each grant will be calculated upon the information you provided in your “Financial Support justification” document, and taking into account the country of origin.

Best regards,

Juan F. Asturiano

Second announcement TS Valencia
Tentative program

First announcement

Dear colleagues,

The AQUAGAMETE COST Action (aquagamete.webs.upv.es) is pleased to announce the 5th AQUAGAMETE Training School.

The 5th AQUAGAMETE Training School is aimed towards young researchers (PhD students, young post-docs and MSc students). The course will be focused on Cryopreservation of fish germ cells, and will include theoretical and practical classes, plus several technical visits. The tentative program is attached.

The course will take place between 7th – 11th March 2016 at the Universitat Politècnica de València (Spain) and PROISER R+D (Spain) facilities, and will be organized by the Group of Aquaculture and Biodiversity of the Institute for Animal Science and Technology (ICTA-UPV) and PROISER´s team.

The number of participants will be restricted to 21 persons, 13 of which being ESRs belonging to the Marie Curie Innovative Training Network IMPRESS, who are also members of the AQUAGAMETE community and will attend the 2nd IMPRESS Workshop in Valencia the previous week. The remaining 8 trainees will be selected by the local organizers among those candidates who send their applications.

The application deadline is 5th February, 2016. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by February 10th. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com).

Financial support from the AQUAGAMETE COST Action (for travelling, accommodation and meals) will be available for students. Registration (100 euros fee) will be available until the 20th February and will include all lunches, access to lectures and laboratorial practices, support material and technical visits.

Applications should include the following documents:

• Application form;
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (explaining how much you expect to spend in travel, accommodation and meals during the course).

More information available soon at: http://aquagamete.webs.upv.es

Best regards,

Juan F. Asturiano

Files to download:
First announcement TS Valencia
Tentative Program TS Valencia
Application form TS Valencia