Cryopreservation of fish germ cells
Valencia (Spain), 7th-11th March, 2016
The 5th AQUAGAMETE Training School took place from 7th to 11th of March, 2016, in the facilities of the Universitat Politècnica de València and PROiSER S.L. (Spain). The course was organized by Juan F. Asturiano, Chair of this Action, and his group. A total of 21 trainees from 10 different countries attended the course, plus some local students who attended as auditors.
Theoretical lectures dealt about different topics related to cryopreservation and were given by 9 experts. Practical classes aimed to train students on techniques for sperm cryopreservation, microinjection of embryos and larvae, isolation of spermatogonia, and sperm quality evaluation by CASA.
Two technical visits were performed, one to the Albufera lagoon and surroundings, where participants enjoyed a boat ride with stops to visit the Tancat de la Pipa (environment preservation center), the Center for Preservation of Freshwater Species (Generalitat Valenciana) and the fishermen facilities in El Palmar, where they were invited to a talk about European eel fishing in Albufera lagoon. After a typical lunch in the village, they visited the Estany, Gola Pujol and Saler beach (natural exit way of migratory eels). The second technical visit was to PROiSER S.L., a company focused on research and development of automatic diagnostic systems, especially for sperm quality evaluation. There, participants were taught about CASA system and its use.
Juan F. Asturiano
You can see the videos and presentations by clicking on the link below the title of each talk.
Monday, March 7th
09:00-09:30 Registration and welcome
09:30-10:00 Progress, challenges and perspectives on fish gamete cryopreservation. J.F. Asturiano (UPV, Spain).
Presentation: Slides Juan F Asturiano
10:00-10:30 Basic info on cryopreservation process. Á. Horváth (SIU, Hungary).
Presentation: Slides Ákos Horváth
10:30-11:00 Coffee break.
11:00-11:30 Surrogate production technology in fish. M. Psenicka (USB, Czech Republic).
Presentation: Slides Martin Psenicka
11:30-12:25 IMPRESS ESRs presentations (7 x 8 min).
12:40-13:45 IMPRESS ESRs presentations (8 x 8 min).
Presentation: Slides Elia Ciani
15:00-18:00 Practical 1: Sperm cryopreservation in marine fish. David S. Peñaranda + UPV team.
Tuesday, March 8th
09:00-18:00 Technical visits:
Puerto de Catarroja,
Tancat de la Pipa,
Center for preservation of freshwater species CV (CCEDCV)-El Palmar,
Fishermen facilities El Palmar and talk by M. Jover (UPV, Spain): Eel fishing in Albufera lake.
Estany, Gola Pujol and Saler beach.
Wednesday, March 9th
08:15-09:00 Somatic cells as an additional resource for genome preservation: the promises and the difficulties. C. Labbé (INRA, France).
Presentation: Slides Catherine Labbé
09:00-11:00 Practical 2, part I: Microinjection of ZF embryos. M. Psenicka (USB, Czech Republic) + UPV team.
11:00-11:30 Coffee break.
11:30-13:00 Practical 2, part II: Transplantation of blastomeres from injected zebrafish embryos M. Psenicka (USB, Czech Republic) + UPV team.
14:30-15:15 Epigenetics and non-Mendelian inheritance. F. Piferrer (CSIC, Spain).
15:15-16:00 Fish PGC cryopreservation: is it a realistic tool for fish conservation biology? V. Robles (IEO, Spain).
Presentation: Slides Vanesa Robles
Thursday, March 10th
09:00-12:00 Practical 3: Isolation and transplantation of sturgeon spermatogonia into sturgeon larvae. M. Psenicka (USB, Czech Republic) + UPV team.
12:00-12:30 Coffee break.
12:30-13:15 Standardization and reporting in cryopreservation. Á. Horváth (SIU, Hungary).
Presentation: Slides Akos Horvath Standardization
13:15-13:45 Case study 1: Gonia cryopreservation (sturgeon). M. Psenicka (USB, Czech Republic).
15:15-15:45 Case study 2: European eel sperm cryopreservation. L. Pérez (UPV, Spain).
Presentation: Slides Luz Pérez
15:45-16:15 Case study 3: Salmon sperm cryopreservation (Efficient method for cryopreservation of salmonid fish semen). A. Ciereszko (PAN, Poland).
Presentation: Slides Andrzej Ciereszko
16:15-17:05 AQUAGAMETE ESRs presentations
Presentations: Slides Sude Atmaca, Slides Pietro Boccaletto, Slides Jorge Hernández Urcera, Slides Eszter Kasa, Slides Agnes Osz, Slides Taina Rocha Almeida, Slides Momin Siddique
Friday, March 11th
09:00-11:00 Technical visit PROISER facilities. C. Soler and PROISER team (PROISER, Spain).
11:00-11:30 Coffee break.
11:30-13:30 Practical 4: Sperm quality evaluation (CASA). C. Soler and PROISER team (PROISER, Spain) / AQUAGAMETE Core Group + WG2 meetings.
13:30-15:00 Lunch (UV Campus).
Valencia, January 18th, 2016
More detailed information is now available for the 5th AQUAGAMETE Training School which will be held at the Universitat Politècnica de València and PROiSER R+D facilities, Spain.
Please visit http://trainingschoolvalencia.weebly.com/, where you will find specific and practical information about the event, including a link to the registration form.
We take the chance to remind you that the application deadline is February 5th. Complete applications must be sent via e-mail to email@example.com. Eligible candidates will be announced by February 10th. Please do not register until you have been notified that were selected as trainee. For more detailed information please contact Pepa Bayarri (firstname.lastname@example.org). Applications should include:
- Application form.
- Short Curriculum Vitae in the area.
- Motivation letter for participating in the training school, addressed to Dr. Juan F. Asturiano and indicating the academic/professional status of the applicant.
- Letter of recommendation from the applicant’s supervisor or employer.
- Financial Support justification (brief explanation of expected travel costs derived of the attendance to the Training School, especially in travel, accommodation and meals).
Please make sure that your name is present in every document.
Financial support from the AQUAGAMETE COST Action (for travelling, accommodation and meals) will be available for selected trainees. The amount of each grant will be calculated upon the information you provided in your “Financial Support justification” document, and taking into account the country of origin.
Juan F. Asturiano
The AQUAGAMETE COST Action (aquagamete.webs.upv.es) is pleased to announce the 5th AQUAGAMETE Training School.
The 5th AQUAGAMETE Training School is aimed towards young researchers (PhD students, young post-docs and MSc students). The course will be focused on Cryopreservation of fish germ cells, and will include theoretical and practical classes, plus several technical visits. The tentative program is attached.
The course will take place between 7th – 11th March 2016 at the Universitat Politècnica de València (Spain) and PROISER R+D (Spain) facilities, and will be organized by the Group of Aquaculture and Biodiversity of the Institute for Animal Science and Technology (ICTA-UPV) and PROISER´s team.
The number of participants will be restricted to 21 persons, 13 of which being ESRs belonging to the Marie Curie Innovative Training Network IMPRESS, who are also members of the AQUAGAMETE community and will attend the 2nd IMPRESS Workshop in Valencia the previous week. The remaining 8 trainees will be selected by the local organizers among those candidates who send their applications.
The application deadline is 5th February, 2016. Complete applications must be sent via e-mail to email@example.com. Eligible candidates will be announced by February 10th. For more detailed information please contact Pepa Bayarri (firstname.lastname@example.org).
Financial support from the AQUAGAMETE COST Action (for travelling, accommodation and meals) will be available for students. Registration (100 euros fee) will be available until the 20th February and will include all lunches, access to lectures and laboratorial practices, support material and technical visits.
Applications should include the following documents:
• Application form;
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (explaining how much you expect to spend in travel, accommodation and meals during the course).
More information available soon at: http://aquagamete.webs.upv.es
Juan F. Asturiano