Molecular basis of fish gamete quality: genomic tools
Rennes (France) 6th-10th June, 2016

Scientific report

The 6th Aquagamete training school was held in Rennes from June 6th to 10th, 2016, on the Rennes 1 University campus of Beaulieu. It was organized by Julien Bobe, leader of the WG1. Techniques for evaluation of gametes quality, and Catherine Labbé. The trainers included 11 staff from the INRA Fish Physiology and Genomics lab, for both the practical and the conferences, and two trainers from Spain, Dr Paz Herraez and Dr Francesc Piferrer. The training course welcomed 18 trainees from 11 countries. It was focused on the Molecular Basis of gamete quality and reproduction, with a strong orientation towards GENOMIC TOOLS. The 2h conferences spanned over sperm genetic damage and repair in fish, epigenetics and inheritance, microRNA and female fecundity, and insight into several genomic tools including the sequencing technologies, and transgenesis approaches with a special focus on CrispR/Cas9. The practical sessions were focused on RNA extraction and quality checking, microarray analysis of fish samples in reproduction research, and sequence searching including tools presentation, primer design, phylogeny/syntheny search. A visit of the INRA experimental fish facility allowed the trainees to discover the fully equipped behavior room, with the test chambers and the video recording system, and the rearing rooms of more than 7 freshwater species with specific photoperiod, water parameters, and temperature. The functioning of the water recycling system was explained, together with the anti-escapees system required for the transgenic fish of the facility (trout, medaka, goldfish, zebrafish). The dedication of the trainers and the motivation of the trainees ensured the success of this intense week of training.

Second annuncement

Rennes 26th April 2016

Dear Colleagues,

The 6^th AQUAGAMETE training school will be focused on the Molecular Basis of gamete quality and reproduction, with a strong orientation towards GENOMIC TOOLS. It will be held in Rennes, at the Fish Physiology and Genomics Department of INRA.

Deadline for application is May 2^nd 2016, do not miss it.

Eligible candidates will be announced by May 6th.

They will be provided with a list of hotels nearby the lab, or in the city center. The course will take place at INRA on the Biology campus of BEAULIEU (Allée Henri Fabre), 15 min from the city center by bus (30 min by foot).

Because of a tight schedule during the course, participants should plan to arrive on Sunday 5^th, and to leave on Saturday 11^th.

The course will include conferences (2h each) on:

• Sperm genetic damage and repair in Fish (Paz Herraez, Spain)
• Overview of sequencing technologies and strategies available to analyze fish reproduction (Yann Guiguen, France)
• Epigenetics and inheritance (Francesc Piferrer, Spain)
• Transgenesis approaches in fish reproduction (Violette Thermes, France)
• CrispR/Cas9 technology for research on fish reproduction (Amaury Herpin, France)
• microRNA and female fecundity (Amine Bouchareb, France)

The practical sessions will be coordinated by the Fish Physiology and Genomics staff. It will consist in experiment on:

• RNA extraction and quality checking (3h30)
• Microarray analysis of fish samples in reproduction research (10h)
• Sequence searching including tools presentation, primer design, phylogeny/syntheny search (5 h)

Financial support from the AQUAGAMETE COST action for travelling, accommodation and meals will be available for students. The access to lectures, laboratory facilities and support material is supported for all participants by the AQUAGAMETE COST action and by the host Fish Physiology and Genomics department.

Complete applications must be sent via e-mail to aquagamete@gmail.com. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com).

Applications should include the following documents:

• Application form
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (only for students).

Information available at: https://aquagamete.webs.upv.es

Location of the training course (Allée Henri Fabre, Campus de Beaulieu, Rennes) available at https://www.google.fr/maps/@48.1182969,-1.6413958,17z

Best regards,

Julien Bobe and Catherine Labbé

Downloadable files:
2nd announcement 6th Aquagamete training school
Application form TS Rennes 2016

First announcement

Rennes 4th April 2016

Dear Colleagues,

The AQUAGAMETE COST ACTION is pleased to announce the 6th AQUAGAMETE training school which will be focused on the Molecular Basis of gamete quality and reproduction, with a strong orientation towards GENOMIC TOOLS.

The course will be organized by the Fish Physiology and Genomics Department of INRA, Rennes (France) (coordination by Julien Bobe and Catherine Labbé), and will be held in Rennes from June 6th to 10th 2016.

The course will include conferences on:
– Sperm genetic damage and repair in Fish (Paz Herraez, Spain)
– Overview of sequencing technologies and strategies available to analyze fish reproduction (Yann Guiguen, France)
– Epigenetics and inheritance (Francesc Piferrer, Spain)
– Transgenesis approaches in fish reproduction (speaker to be determined)
– CrispR/Cas9 technology for research on fish reproduction (Amaury Herpin, France)
– microRNA and female fecundity (Amine Bouchareb, France)

The practical sessions will be coordinated by the Fish Physiology and Genomics staff. include experiment on:
– RNA extraction (2h30)
– Microarray analysis of fish samples in reproduction research (10h)
– Sequence searching including tools presentation, primer design, phylogeny/syntheny search (5 h)

The number of participant is restricted to 16 persons. Priority will be given to early stage researchers (MSc students, PhDstudents, young post-docs) and to participants with no experience in genomics and with a professional project related to gamete quality or fish reproduction, including representative from industry.

Financial support from the AQUAGAMETE COST action for travelling, accommodation and meals will be available for students. The access to lectures, laboratory facilities and support material is supported for all participants by the AQUAGAMETE COST action and by the host Fish Physiology and Genomics department.

The application deadline is May 2nd, 2016. Complete applications must be sent via e-mail to aquagamete@gmail.com. Eligible candidates will be announced by May 6th. For more detailed information please contact Pepa Bayarri (aquagamete@gmail.com).

Applications should include the following documents:
• Application form (downloadable below);
• Short Curriculum Vitae in the area;
• Motivation letter for participating in the training school, which should indicate the academic/professional status of the applicant;
• Letter of recommendation from the applicant’s supervisor or employer;
• Financial Support justification (only for students).

More information available soon at: https://aquagamete.webs.upv.es

Best regards

Julien Bobe and Catherine Labbé

Downloadable files:
First announcement TS Rennes 2016
Application form TS Rennes 2016