{"id":394,"date":"2015-09-15T08:45:16","date_gmt":"2015-09-15T08:45:16","guid":{"rendered":"http:\/\/aquagamete.webs.upv.es\/aquagameteblog\/?p=394"},"modified":"2015-09-15T08:45:16","modified_gmt":"2015-09-15T08:45:16","slug":"assistance-for-creating-a-database-of-fish-ribosomal-rna","status":"publish","type":"post","link":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/assistance-for-creating-a-database-of-fish-ribosomal-rna\/","title":{"rendered":"Assistance for creating a database of Fish Ribosomal RNA"},"content":{"rendered":"

\"Universidad<\/a>
\nDear colleagues,<\/p>\n

We are writing to you with the intention to seek your help and assist in the development of a database about:<\/p>\n

The Profiling Of Fish Gonadal Ribosomal RNA<\/span><\/p>\n

In the BCTA research group of the Department of Zoology and Cell Biology at the University of the Basque Country(UPV\/EHU), we have been workin g in the field of ecotoxicology several years using various models of fish. In the most\u00a0recent years we have tried to explain the usefulness of a new methodology for sexing fish which is based on\u00a0ribosomal RNA electrophoretic-migration obtained by extraction of total RNA from gonad (Diaz de Cerio et al, 2012).
\nThe 5S rRNA is more abundant in females than in males, so a simple electrophoresis helps us identifying sex without\u00a0any histological process. We can also identify the female reproductive stage making a simple index of 5S \/ 18S rRNA\u00a0that identifies previtellogenesis females, cortical alveoli, vitellogenesis or maturing stage. Moreover, the same\u00a0analysis distinguishes intersex males (males with oocytes in testis) by the fact that the 5S rRNA is abundantly\u00a0expressed in oocytes.
\nIn our laboratory we have tested the technique in commercial species that commonly inhabit our coast: hake,\u00a0flounder, anchovy, sardine, horse mackerel and mackerel (among others). To generalize the use of this methodology\u00a0to other species, we need to prove that it is useful to fish species with different strategies and reproductive cycles.
\nAn additional problem is that we lack intersex individual samples in species other than Chelon labrosus.
\nRecently, in the “5th International Workshop on the Biology of Fish Gametes 2015” held in Ancona (Italy), we have\u00a0called for collaboration between laboratories to collect XDA files with the rRNA profiles of gonads of different\u00a0species. These files are the results of the quality analysis of RNA using as an instrument the Agilent 2100 Bioanalyzer.
\nTherefore, we will be deeply grateful if you could participate in our initiative with your stored files and completing\u00a0the information we require.
\nIn the following link we explain the process in more detail, and you can download additional information and an\u00a0explanatory video.
\nhttps:\/\/www.researchgate.net\/post\/Does_anyone_want_to_participate_in_a_Collaborative_Network_about_Profiling_of_Fish_Gonadal_Ribosomal_RNA
\nThe whole process should not take you more than 20 minutes.
\nTo thank your help and the possible obtained results, we hope to write a scientific article in which a member of each\u00a0research group would be invited to participate.
\nIf you have any doubts, do not hesitate to contact us using the following contact fishoorna@gmail.com. Or just\u00a0leave a question in the link provided above.<\/p>\n

Yours faithfully,<\/p>\n

The BCTA research group
\n\"Universidad<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Dear colleagues, We are writing to you with the intention to seek your help and assist in the development of a database about: The Profiling Of Fish Gonadal Ribosomal RNA In the BCTA research group of the Department of Zoology and Cell Biology at the University of the Basque Country(UPV\/EHU), we have been workin g […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-394","post","type-post","status-publish","format-standard","hentry","category-sin-categoria"],"_links":{"self":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/394","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/comments?post=394"}],"version-history":[{"count":2,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/394\/revisions"}],"predecessor-version":[{"id":398,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/394\/revisions\/398"}],"wp:attachment":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/media?parent=394"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/categories?post=394"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/tags?post=394"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}