{"id":229,"date":"2013-10-10T09:02:59","date_gmt":"2013-10-10T09:02:59","guid":{"rendered":"http:\/\/aquagamete.webs.upv.es\/aquagameteblog\/?p=229"},"modified":"2013-10-16T12:29:36","modified_gmt":"2013-10-16T12:29:36","slug":"two-new-publications-in-fugu","status":"publish","type":"post","link":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/two-new-publications-in-fugu\/","title":{"rendered":"Two new publications in fugu"},"content":{"rendered":"

Two articles have been published in Aquaculture:<\/p>\n

\"Imagen1\"<\/a>\u00a0Photo by V\u00edctor Gallego.<\/p>\n

V. Gallego, L. P\u00e9rez, J.F. Asturiano, M. Yoshida.\u00a0Study of pufferfish (Takifugu niphobles) sperm: Development ofmethods\u00a0for short-term storage, effects of different activation media and role of\u00a0intracellular changes in Ca2+ and K+ in the initiation of sperm motility.\u00a0\u00a0Aquaculture 414\u2013415 (2013) 82\u201391.
\nAbstract: The first goal of this study was the development of a short-term storage method for pufferfish (Takifugu\u00a0niphobles) sperm. In this respect, the best results were obtained by diluting the sperm in a seminal-like solution\u00a0and keeping it in a Petri dish in chilled storage (4 \u00b0C). This method was successful in preserving sperm quality\u00a0parameters without resulting in differences in fresh sperm for a relatively long-term period (7 days), for use in\u00a0aquaculture matters. The addition of bovine serum albumin (BSA) to the medium did not improve the results.\u00a0On the other hand, both the osmolality and the ion composition of themedia are essential factorswhich canmodulate\u00a0the sperm motility parameters. The osmolality of the activating medium was the most important factor in\u00a0triggering pufferfish sperm motility, and osmolalities of 750\u2013825 mOsm\/kg were necessary to initiate this process,\u00a0demonstrating that it appears to be a dose-independent mechanism. Regarding the ion composition of\u00a0the activationmedia, this study has shownthat despite the spermatozoa being able to initiatemovementwithout\u00a0any ion in the activationmedium, the absence of ions can negatively affect the kinetic parameters of the spermatozoa.\u00a0Finally, in natural conditions (seawater), the activation of sperm motility generates intracellular increases\u00a0in Ca2+ and K+, suggesting that these ions play an essential role in the activation mechanismof pufferfish sperm.<\/p>\n

\"Exposure:\u00a0<\/a>Photo by V\u00edctor Gallego.<\/p>\n

V. Gallego, L. P\u00e9rez, J.F. Asturiano, M. Yoshida.\u00a0Relationship between spermatozoamotility parameters, sperm\/egg ratio,\u00a0and fertilization and hatching rates in pufferfish (Takifugu niphobles).\u00a0Aquaculture 416-417 (2013) 238\u2013243.
\nAbstract:\u00a0The use of high quality gametes from both males and females during in vitro fertilization (IVF) trials is an essential\u00a0step in order to achieve high fertilization and hatching rates. Although aquaculture hatcheries have focused\u00a0more on egg rather than spermatozoa quality, some studies have demonstrated that sperm quantity and quality\u00a0have a great influence both on fertilization\/hatching success and the subsequent development of the embryo and\u00a0larvae.\u00a0In this study we have demonstrated that sperm\/egg ratio and sperm quality are factors strongly related to each\u00a0other in the pufferfish (Takifugu niphobles). Our results suggest that both factors should be taken into account as\u00a0unique interrelated elements,making possible to obtain high fertilization rates using a successful combination of\u00a0small amount of high quality sperm or high amount of low quality sperm.\u00a0In addition, coefficients of correlation and determination among all the sperm motion parameters provided by a\u00a0CASA systemand fertilization\/hatching rateswere estimated for the first time in a marine species. Positive significant\u00a0correlationswere found in someparameters such as total and progressivemotility (0.68 and 0.7 respectively).\u00a0However, curvilinear velocity (VCL), straight line velocity (VSL) and average velocity (VAP) showed the\u00a0highest coefficients of correlation (0.82, 0.8, and 0.81, respectively). In this respect, spermatozoa velocity appears\u00a0to be a key factor in the fertilization process, especiallywhen the number of spermatozoa per egg is limited in the\u00a0aqueous environment.<\/p>\n

\n","protected":false},"excerpt":{"rendered":"

Two articles have been published in Aquaculture: \u00a0Photo by V\u00edctor Gallego. V. Gallego, L. P\u00e9rez, J.F. Asturiano, M. Yoshida.\u00a0Study of pufferfish (Takifugu niphobles) sperm: Development ofmethods\u00a0for short-term storage, effects of different activation media and role of\u00a0intracellular changes in Ca2+ and K+ in the initiation of sperm motility.\u00a0\u00a0Aquaculture 414\u2013415 (2013) 82\u201391. Abstract: The first goal of […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-229","post","type-post","status-publish","format-standard","hentry","category-sin-categoria"],"_links":{"self":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/229","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/comments?post=229"}],"version-history":[{"count":7,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/229\/revisions"}],"predecessor-version":[{"id":243,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/posts\/229\/revisions\/243"}],"wp:attachment":[{"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/media?parent=229"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/categories?post=229"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aquagamete.webs.upv.es\/aquagameteblog\/wp-json\/wp\/v2\/tags?post=229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}